Figure 5.

The proximal PBE is required for activin A-stimulated transcription. A, LβT2 cells seeded in 24-well plates were transfected with −1990/+1 mFshb-luc (WT) or a similar construct containing PBEmut. The next day, cells were treated with 1 nm activin A or control medium and lysates collected 24 h later. Data from three experiments are presented as fold change in the reporter activity, normalized relative to the untreated WT reporter set to 1. B, As in A, LβT2 cells were transfected with −1028/+7 hFSHB-luc (WT), with the −1028/+7 reporter containing PBEmut, or with empty pGL3-Basic and treated with activin A. Data are derived from three experiments performed in triplicate. Statistical differences are indicated with different letters (Tukey test of significant interaction).