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. Author manuscript; available in PMC: 2009 Jan 8.
Published in final edited form as: Biochemistry. 2007 Dec 15;47(1):479–489. doi: 10.1021/bi7013882

Fig 5. Effect of intact and deleted PRLRs on the growth of human breast cancer T47D cells (A) and human prostate cancer DU145 cells (B) in the absence of added PRL.

Fig 5

Receptor constructs were transfected into either T47D or DU145 cells. Forty eight hours post transfection, the medium with serum was refreshed. After a further 24h, the medium was changed to serum-free DMEM to conduct the MTS assay. Data are expressed as a percent of the control to illustrate the change induced. The data are not corrected for either transfection efficiency or expression efficiency for reasons discussed in the text. The same overall result was obtained when anti-PRL was added to the incubation, although cell number was decreased in each incubation indicative of an effect of the antibody on autocrine PRL. *, significantly different from the intact counterpart with p<0.05.