Table 5.
Genes significantly altered in response to Bacillus anthracis infection in murine hearts at 48 hr
| GenBank ID | Gene Name | Function | FC |
|---|---|---|---|
| Up-regulated in Response to B. anthracis Infection | |||
|
| |||
| Apoptosis | |||
| AV228493 | Interleukin-1 receptor-associated kinase 3 (Irak3) | Cytokine and chemokine mediated signaling pathway; apoptosis | 1.7 |
| NM_010177 | Tumor necrosis factor (ligand) superfamily, member 6 (Tnfsf6); Fas ligand (Fasl) | Apoptosis; immune response | 1.7 |
| Growth, differentiation, and development | |||
| AK004781 | SRY-box containing gene 17 (Sox17) | Regulation of transcription, DNA-dependent; cell differentiation; cardiogenesis | 2.2 |
| Immune response and inflammation | |||
| NM_009921 | Cathelicidin antimicrobial peptide (Camp) | Defense response to bacterium | 1.8 |
| BB030365 | CD8 antigen, alpha chain (Cd8a) | Immune response; cytotoxic T cell differentiation | 1.8 |
| BB530063 | Fc receptor, IgG, low affinity Iib (Fcgr2b) | Negative regulation of type I hypersensitivity;phagocytosis; defense response | 2.1 |
| X03019 | Granulocyte-macrophage colony stimulating factor (GM-CSF) | Immune response; myeloid dendritic cell differentiation | 1.8 |
| NM_008694 | Neutrophilic granule protein (Ngp) | Defense response | 2.3 |
| Iron homeostasis | |||
| BM237750 | Transferrin (Trf) | Iron ion homeostasis | 1.6 |
| Metabolism | |||
| BB503164 | Carbonic anyhydrase 12 (Car12) | One-carbon compound metabolism | 2.4 |
| AV236319 | Carnitine palmitoyltransferase 2 (Cpt2) | Fatty acid metabolism | 2.0 |
| Protein processing | |||
| NM_008456 | Kallikrein 5 (Klk5) | Proteolysis | 2.3 |
| Signal transduction | |||
| AV047570 | Calmodulin 3 (Calm3) | G-protein coupled receptor protein signaling pathway | 3.2 |
| BC019382 | Metallothionein 2A (Mt2a) | Phosphatidylinositol metabolism; Nitric oxide-mediated signaling | 1.9 |
| AV328460 | Tachykinin receptor 3 (Tacr3) | G-protein coupled receptor protein signaling pathway; phosphatidylinositol-calcium second messenger system | 2.0 |
| AV246932 | Regulator of G-protein signaling 2 (Rgs2) | Regulation of G-protein coupled receptor protein signaling pathway | 1.65 |
| Stress response | |||
| BC015260 | FK506 binding protein 5 (51 kDa) (Fkbp5) | Stress response; steroid signaling | 3.2 |
| Transport | 1.7 | ||
| AV323698 | ARP1 actin-related protein 1 homolog A (Actr1a) | Vesicle-mediated transport | 1.9 |
|
| |||
| Down-regulated in Response to B. anthracis Infection | |||
|
| |||
| Adhesion and migration | |||
| NM_009866 | Cadherin 11 (Cdh11) | Cell adhesion | −3.2 |
| BC005676 | Cell surface glycoprotein CD44 (hyaluronate binding protein) (Cd44) | Cell adhesion | −2.3 |
| BF227507 | Procollagen, type I, alpha 2 (Col1a2) | Cell adhesion | −2.3 |
| NM_007737 | Procollagen, type V, alpha 2 (Col5a2) | Cell adhesion | −1.8 |
| NM_011693 | Vascular cell adhesion molecule 1 (Vcam1) | Cell adhesion | −2.7 |
| Angiogenesis | |||
| BB453314 | Angiopoietin 1 (Angtpt1) | Angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; development | −2.1 |
| Growth, differentiation, and development | |||
| BC004724 | Fibronectin 1 (Fn1) | Cell morphogenesis; acute-phase response; cell-substrate junction assembly; cell adhesion | −2.2 |
| NM_009627 | Adrenomedullin (Adm) | Heart development; positive regulation of cell proliferation | −1.7 |
| AI323506 | Myelin basic protein (Mbp) | Myelination; cell differentiation | −1.9 |
| C81601 | Tissue inhibitor of metalloproteinase 2 (Timp2) | Negative regulation of cell proliferation;regulation of cAMP metabolism; regulation of MAPKKK signaling | −1.7 |
| Immune response and inflammation | |||
| L23495 | MHC class I H-2K (H2-K1) | Antigen processing and presentation | −2.0 |
| NM_018866 | Small inducible cytokine subfamily B (Cys-X-Cys), member 13 (Scyb13) (Cxcl13) | Chemotaxis; inflammation | −5.4 |
| AY090098 | Interferon, alpha-inducible protein 27 (Ifi27) | Defense response | −3.1 |
| Iron homeostasis | |||
| AK011596 | Transferrin receptor (Tfrc) | Iron ion homeostasis | −1.7 |
| Metabolism | |||
| BB276877 | ATP citrate lyase (Acly) | Acetyl-CoA biosynthesis; lipid metabolism | −3.4 |
| AF127033 | Fatty acid synthase (Fasn) | Fatty acid biosynthesis | −6.7 |
| NM_009127 | Stearoyl-Coenzyme A desaturase 1 (Scd1) | Lipid biosynthesis; superoxide metabolism | −5.2 |
| Protein processing | |||
| AK009847 | Protease, serine, 23 (Prss23) | Proteolysis | −2.5 |
| Signal transduction | |||
| BB746807 | Adenylate cyclase 7 (Adcy7) | cAMP biosynthesis | −2.1 |
| NM_022881 | Regulator of G-protein signaling 18 (Rgs18) | Regulation of G-protein coupled receptor protein signaling pathway | −3.0 |
| Stress response | |||
| BC024835 | Structure specific recognition protein 1 (Ssrp1) | DNA replication; DNA repair | −1.7 |
| Transcription regulation | |||
| BB124537 | Myotrophin (Mtpn) | Regulation of transcription, DNA-dependent | −1.7 |
| Other | |||
| M12481 | Actin, beta, cytoplasmic (Actb) | Structural constituent of cytoskeleton | −1.9 |
| X53753 | Tropomyosin 3, gamma (Tpm3) | Regulation of muscle contraction | −1.7 |
FC = fold-change
Negative sign (−) before fold-change value indicates down-regulation
Genes shown more than once were represented by more than one probe set on the array, and each was determined separately to be altered in response to Bacillus anthracis treatment