Table 8.

Real-time RT-PCR confirmation of altered genes identified by GeneChip analysis

Gene	Tissue	Time	Affy-metrix Fold Change	RT-PCR Fold Change	Function
Clusterin	Lung	8 hr	2.1	3.98	Molecular chaperone during stress-induced injury.
Angiopoietin-like Protein 4	Lung	48 hr	3.67	2.8	Protects vascularity during inflammation; maintains EC matrix.
Resistin-like Alpha	Lung	48 hr	3.32	6.34	Regulator of apoptosis and glucose uptake in adipose cells.
Amylase 2	Spleen	8 hr	896.46	3847.6	Pancreatic enzyme involved in starch metabolism.
Elastase 3	Spleen	8 hr	403.86	599.19	Pancreatic enzyme involved in breaking down elastin.
T-cell Gamma Variable 4	Spleen	8 hr	−2.4	−1.35	A variable region on the T-cell receptor.
Prostaglandin D2 Synthase	Heart	8 hr	1.7	2.23	Involved in muscle contraction and platelet aggregation.
Transferrin Receptor	Heart	8 hr	−1.87	−1.26	Receptor involved in iron uptake.

FC = fold-change

Negative sign (−) before fold-change value indicates down-regulation

Genes shown more than once were represented by more than one probe set on the array, and each was determined separately to be altered in response to Bacillus anthracis treatment