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. 2008 May 5;7:4. doi: 10.1186/1476-5926-7-4

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Copyright © 2008 Martinez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Production of nitric oxide (NO) and hydrogen peroxide (H₂O₂) by LSEC. Secretion of nitric oxide (NO) was measured in LSEC culture supernatants by Griess reaction at 24 and 48 h (a). Culture media was collected from hyperoxic conditions (open bars) or normoxic conditions (filled bars) at 24 hours intervals. Final concentrations were estimated from individual standard curves. Generation of endogenous H₂O₂was monitored in separate experiments at the indicated time-points in LSEC cultures by H₂O₂-mediated oxidation of DCFH-DA into DFC during 6 h (b). Values are total fluorescence emitted at 545 nm.