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. 2008 Mar 18;14(5):291–299. doi: 10.1093/molehr/gan015

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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

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Figure 5: — Representative micrographs and western blot demonstrating inhibition of oocyte aurora kinases (AURK) results in ser10-histone-H3 hypophosphorylation.

(A) Culture of GV-intact oocytes for 2 h to allow germinal vesicle breakdown(GVBD) in presence of 10 µM ZM447439 resulted in a total lack of ser10-Histone-H3 phosphorylation (d), compared with controls (a). A similar reduction in ser10 phosphorylation was observed following 7 h (MI) of culture in the presence of ZM (j) compared with controls (g). Chromatin was stained with Hoescht and is pictured in blue (b,e,h,k). Overlays are also indicated (c,f,i,l). (B) Western blot analysis confirmed that ZM treatment inhibits ser10 phosphorylation (n = 100 oocytes/lane).