Figure 4. Memory CD8⁺ T cells primed with anti-CD40 produce multiple cytokines.

Mice were primed with PBS or anti-DEC:OVA+anti-CD40, and boosted with anti-DEC:OVA+anti-CD40 at day 60. At the peak of the secondary response (at d 7 after boosting in PBS-primed mice; at d 4 in anti-DEC:OVA+anti-CD40-primed mice), splenocytes were stimulated with OVA(257-264) peptide and the production of IFN-γ, IL-2 and TNF-α were examined by intracellular staining. Representative of two experiments.