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. 2008 Jun;147(2):543–551. doi: 10.1104/pp.108.118307

Figure 2.

Figure 2.

Silencing of FAD2 with 1XsiFAD2. A, Diagrammatic representation of the 1XsiFAD2 construct. Sequence coding for the 3′D2+ siRNA was replaced with base pairs 98 to 118 of the FAD2 CDS coding for the 21-bp siFAD2 sequence (shown in gray). The miR173 target site is shown in black, and sequences encoding other siRNAs are shown in white. In planta expression of the 1XsiFAD2 construct is driven by the CaMV35S promoter. B, Fatty acid analysis of wild type; T1 and T2 transgenic seed harboring the 1XsiFAD2 construct; and T2 transgenic control seed harboring the mut-1XsiFAD2 construct, a version of the 1XsiFAD2 construct in which the miR173 site has been mutated. Data for T1 seed are the average of 10 individual seeds, and data for T2 seed are the average of four individual seeds each from independent families. Fatty acid profiles are expressed as ODP. The sd is shown at the top of each bar.