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. 2008 Jun;147(2):802–815. doi: 10.1104/pp.108.119511

Figure 3.

Figure 3.

Identification of wheat TaMPK6 and TaMPK3 and analysis of their expression during the symptomless phase of incompatible and compatible interactions. A, Candidate wheat homologs of A1 and A2 class plant MAPKs were identified by BLASTP analysis of GenBank and a proprietary wheat EST collection. ClustalW analysis was then performed with the sequences against all 12 Arabidopsis MAPKs containing the TEY activation loop domain (genes At1g10210, At1g59580, At3g45640, At4g01370, At4g11330, At2g43790, At2g18170, At3g59790, At1g01560, At2g46070, At1g07880, and At4g36450) in addition to A1 and A2 class MAPKs from rice (GenBank accession nos. NP_001056846 and ABH01189), tobacco (AAB58396 and BAA09600), and parsley (Petroselinum crispum; AAN65179 and AAN65181/CAA73323). Arrows indicate the positions of the TaMPK6 and TaMPK3 homologs. B, Real-time SYBR Green RT-PCR analysis of TaMPK3 and TaMPK6 expression at 1, 4, and 8 d after inoculation of Cadenza wheat with either isolate IPO323 (incompatible [inc]) or isolate IPO88004 (compatible [comp]).