Figure 7.

Characterization of the ADH1-LUC reporter construct. A, Schematic map of ADH1 promoter and firefly luciferase reporter gene fusion. Position of the transcription start is +1, whereas the position of promoter junction is +39. B, Temporal changes of bioluminescence in the parental ADH1-LUC line during treatments with 200 mm NaCl, 400 mm Suc, and 20 μm ABA for 2, 4, and 12 h. C, Time kinetics of luciferase-mediated light emission in the ADH1-LUC line during treatments with 20 μm ABA, 400 mm Suc, 200 mm NaCl, and 10 mm H₂O₂. Values of total light emission were recorded at every 30 min throughout a period of 18 h. Relative luminescence values are shown, where 1 is the luminescence of untreated control at time 0.