Table I.
FRET-FLIM analysis of interactions between AtEB1a:GFP (S65T) or GFP:MAP4-MBD and MP:RFP (mRFP1) upon transient expression in N. benthamiana leaf cells
The average fluorescence lifetime (τ) values and their respective sds, as determined for AtEB1a:GFP alone or in the presence of MP:RFP, are shown. From the fluorescence lifetimes, the percentage of FRET was calculated by the equation given in “Materials and Methods.” The total number of microtubules analyzed is indicated by n, and N is the number of independent experiments.
| Proteins | Lifetime τ | sd | FRET Efficiency | N | n |
|---|---|---|---|---|---|
| ns | % | ||||
| AtEB1a:GFP | 2.37 | 0.06 | – | 3 | 170 |
| AtEB1a:GFP + MP:RFP | 1.89 | 0.13 | 21 | 3 | 166 |
| GFP:MAP4-MBD | 2.20 | 0.07 | – | 2 | 123 |
| GFP:MAP4-MBD + MP:RFP | 2.21 | 0.07 | 0 | 2 | 179 |