Figure 2.

Reciprocal grafting between wild-type and miR399b-overexpressing transgenic Arabidopsis plants. A, Pi concentration in the scions. Each spot indicates one data point from an independent grafted plant. B, Relative levels of PHO2 mRNA in the scions (black bars) and rootstocks (gray bars) of grafted plants. C and D, Measurement of pri-miR399b (C) and mature miR399b/c (D) in scions (black bars) and rootstocks (gray bars), respectively. The level of mature miR399b/c in wild-type self-grafted plants subjected to 1- or 2-d Pi starvation treatment were included in D. Because of the identical sequences, the level of mature miR399b and c cannot be distinguished. The error bars represent sd (n = 4–5 independent grafted plants). ND, Nondetectable. The difference between two dashed lines in D indicates the potential amount of moved miR399b.