Figure 7.

Native PAGE and immunoblot analysis of protein fractions containing cytosolic proteins (lane 1), solubilized plasma membrane proteins (lane 2), and purified NAD(P)H oxidoreductase protein (lane 3). Amounts of samples giving the same activity of XTT reduction (10 nmol XTTH₂ min⁻¹ for A and 30 nmol XTTH₂ min⁻¹ for B and C) were loaded on the gel. A, The gel was stained for enzyme activity using the NBT assay. B and C, Immunoblots were decorated with antibodies directed against NQR and FQR1.