TABLE 1.
EGF | − | + | − | + | ||||
Epinephrine | − | − | + | + | ||||
Day in culture | 2 | 6 | 2 | 6 | 2 | 6 | 2 | 6 |
P2X7 receptor forms (kDa) | ||||||||
85 | 10 | 80 ± 15a | 10 ± 5 | 80 ± 5 | 10 ± 5 | 20 ± 5b | 10 ± 5 | 5 ± 2b |
75 | 10 ± 3 | |||||||
65 | 10 | 3 ± 2 | 10 ± 2 | 3 ± 2 | 5 ± 2b | 1 ± 1b | 20 ± 5 | 1 ± 2b |
18 | 10 | 70 ± 10 | 10 ± 5 | 110 ± 10b | 10 ± 5 | 150 ± 25b | 500 ± 15b | 350 ± 30b |
Data are means (± SD) of densitometry analyses of three experiments (see Fig. 5).
Arbitrary densitometric values normalized to the expression of the various P2X7 receptor forms in d-2 cells not treated with EGF or epinephrine.
P < 0.05–0.01 compared with bands’ densities in d-2 cells not treated with EGF or epinephrine.