FIG. 1.
ET-1 lowers plasma membrane PIP2 and impairs insulin action. 3T3-L1 adipocytes were exposed to 10 nmol/l ET-1 for the indicated times and left untreated (A) or treated (B) with 10 nmol/l insulin for 30 min. Representative plasma membrane PIP2 (A) and GLUT4 (B) images from three experiments are shown. IF, immunofluorescence; PM, plasma membrane.