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PIP3 corrects defects in Akt-1 but not GLUT4 or actin. PIP3 add-back was performed as described for PIP2 add-back in Fig. 6. Shown are cell PIP3 (A), membrane GLUT4 (C), cell F-actin (D), and a phosphoserine Akt immunoblot (B). All data shown are representative from 3–5 experiments. IF, immunofluorescence; PM, plasma membrane.
