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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: J Struct Biol. 2007 Aug 11;161(3):232–242. doi: 10.1016/j.jsb.2007.08.002

Figure 3.

Figure 3

The tilt axis angle is determined by running one iteration of tracking with a range of tilt axis angles, and choosing the angle giving the smallest residual. The fiducial marker radii for tilt series taken at different magnifications were (A) 10 pixels (54500x), (B) 7 pixels (38500x), and (C) 5 pixels (26000x). The width of the inverse peak expressed as the sigma coefficient of a Gaussian curve fitted to the profile (broken lines in A–C), is a function of the marker radius (D), determining how accurately the tilt axis angle must be specified for successful tracking.