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Comparison of the reconstruction of the same gold fiducial marker using (A) the weighted backprojection in IMOD (Kremer et al., 1996) and (B) the reciprocal space algorithm in Bsoft, and with (C) the gold particle image removed (“painted over”) before reconstruction. In each panel, the top left shows the xy plane, the top right shows the yz plane, and the bottom left shows the xz plane (which is approximately perpendicular to the tilt axis).
