High resolution carbon spectra calibrated and normalized to the CH peak at 285 eV. (A) blank silicon nitride, (B) silicon nitride with ethanolamine HCl, (C) silicon nitride with ethanolamine HCl and NHS-PEG-PDP, (D) silicon nitride with NHS-PEG-PDP, and (E) silicon nitride with ethanolamine HCl, NHS-PEG-PDP, and ScpB. The peak at 285 eV is due to hydrocarbon C-C/C-H, the peak at 287 eV is due to C-N/C-O, and the peak at 289 eV is due to O-C=O/N-C=O. Shake up satellites from the pyridine ring are observed near 293.5 eV.