Figure 3.

Sialic acid-dependent IgG anti-inflammatory activity is linkage specific, although the reduced antibody dependent cytotoxicity is not. A. Anion exchange purified sialylated IVIG Fcs were administered to mice 1 hour prior to K/BxN sera, and paw swelling monitored over the next several days. Means and standard deviations of 4 mice per group are plotted; *denotes p<0.05 as determined by Anova followed by Tukey’s post hoc. To determine whether the reduced ADCC of sialylated antibodies was also dependent on specific linkages, platelet-depleting 6A6-IgG2b antibodies were sialylated as described above. (B) Sialylated 6A6-IgG2b antibodies were administered to mice and platelet counts determined 0, 4, and 24 hours following treatment. Mean and standard deviation of 5 mice per group are plotted; *p<0.05 as determined by Anova followed by Tukey’s post hoc.