Fig. 4.

Ocular and tumor immunity is associated with IFN-γ and the up-regulation of MHC class I. (A) In situ detection of pmel-1, IFN-γ, and MHC class I in the eye after ACT using confocal microscopy. (B) Detection of enhanced MHC class I expression in tumor 5 days after ACT of pmel-1 using confocal microscopy. Images are representative of multiple fields. (C) The induction of ocular autoimmunity was blindly evaluated 5 days after the adoptive transfer of WT, FasL, TNF-α, perforin or IFN-γ-deficient pmel-1 T cells in WT tumor-bearing mice. (D) Tumor growth was assessed after the ACT of pmel-1 WT T cells (●) or pmel-1 T cells deficient in IFN-γ (▴), perforin (▵), FasL (♦), TNF-α (■), or no cells (○). Data are representative of two independently performed experiments.