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. 2006 Dec 13;23(6):486–498. doi: 10.1080/09687860600841967

Figure 4.

Figure 4

R306E-MCT1 and E218R-basigin are not expressed at the plasma membrane of Xenopus oocytes. Oocytes were micro-injected with the cRNA shown and after 72 hours some oocytes were used for immunofluorescence microscopy with the antibody shown (panel A) and others used for membrane preparation followed by SDS-PAGE (20 mg protein) and western blotting with anti-rat MCT1 antibody (panel B). For the western blot, kidney plasma membranes were used as a positive control. The faint band in the water-injected controls represents very slight sample contamination and is only visible because of the over-exposure of the blot to ensure any expressed MCT was detected. Further details are given under ‘Methods’. This Figure is reproduced in colour in Molecular Membrane Biology online.