Table 1.
Semi-quantitative measurement of c-fos mRNA induction in various brain regions across varying intensities of noise, reported as mean integrated gray values (/100 ± SEM)
| Brain region | Control | 80 dB | 85 dB | 90 dB | 95 dB | 100 dB | 105 dB | 110 dB |
|---|---|---|---|---|---|---|---|---|
| Forebrain | ||||||||
| BNST medial* | 0.9 (0.3) | 8.3a (1.6) | 36a (6) | 51b (7) | 89b (8) | 103b (11) | 134b (13) | 167b (15) |
| BNST ventral* | 2.3 (1.0) | 5.0a (1.7) | 13a (5) | 24b (8) | 46b (18) | 79c (25) | 91c (23.4) | 64b (8) |
| Caudate nucleus dor* | 3.3 (0.9) | 14a (2) | 31a (15) | 43b (10) | 69b (6) | 111c (18) | 87b (23) | 130c (24) |
| Caudate nucleus ven* | 1.2 (0.4) | 2.9 (1.1) | 6.4a (4.7) | 6.8a (2.0) | 13b (2) | 13b (0.3) | 15b (3) | 30c (4) |
| Medial septum | 0.6 (0.3) | 2.3 (1.4) | 1.0 (0.4) | 3.5 (1.8) | 2.3 (1.1) | 4.8 (2.1) | 4.6 (1.2) | 5.7 (2.6) |
| Ventrolateral septum* | 8.3 (2.1) | 18 (5) | 87a (20) | 125b (78) | 598c (181) | 527c (125) | 497c (101) | 578c (113) |
| Septohypothal. nuc* | 4.6 (2.6) | 8.3a (1.6) | 37a (6) | 52b (7) | 498b (81) | 517b (149) | 453b (98) | 503b (54) |
| Amygdala | ||||||||
| Central nucleus | 0.9 (0.2) | 2.7 (1.5) | 2.1 (1.0) | 3.1 (1.9) | 1.2 (0.6) | 3.4 (1.2) | 3.1 (0.9) | 2.7 (1.5) |
| Basolateral nucleus | 0.8 (0.1) | 1.7 (0.4) | 5.1 (2.3) | 1.3 (0.2) | 2.9 (1.0) | 2.3 (0.8) | 5.4 (2.1) | 2.9 (1.2) |
| Lateral nucleus* | 1.2 (0.6) | 4.5a (2.1) | 7.1a (3.2) | 11a (4) | 27b (7) | 14a (3) | 17b (3) | 21b (4) |
| Medial nucleus | 1.1 (0.4) | 3.1 (0.9) | 2.5 (1.0) | 6.9 (2.8) | 6.8 (2.1) | 5.5 (1.2) | 7.3 (3.2) | 6.4 (2.0) |
| Hippocampus | ||||||||
| CA1 (dorsal) | 0.7 (0.2) | 3.9 (1.7) | 3.4 (1.6) | 2.3 (0.5) | 3.7 (0.7) | 5.3 (1.0) | 4.0 (0.7) | 5.0 (1.0) |
| CA3 (dorsal) | 7.4 (2.9) | 14 (2.8) | 9.4 (3.0) | 13 (2.8) | 12 (4.3) | 18 (3.6) | 19 (4.0) | 16 (3.9) |
| Dentate gyrus (dorsal) | 6.3 (0.4) | 6.8 (1.7) | 6.6 (2.5) | 7.8 (1.9) | 6.8 (0.5) | 7.7 (1.1) | 8.2 (1.1) | 8.8 (1.4) |
| Subiculum dorsal* | 6.1 (1.2) | 10 (2) | 8.1 (1.3) | 10 (3) | 17a (5) | 23a (4) | 12 (1) | 19a (3) |
| Subiculum anteroventral* | 2.7 (0.8) | 5.2 (1.1) | 4.6 (0.7) | 46b (5) | 63b (19) | 76b (11) | 59b (4) | 73b (8) |
| Cortex | ||||||||
| Frontal | 0.7 (0.2) | 2.0 (0.2) | 7.5 (3.7) | 7.4 (1.3) | 7.6 (2.3) | 16 (4.6) | 7.5 (2.8) | 9.6 (5.2) |
| Infralimbic | 0.6 (0.1) | 8.7 (6.7) | 8.4 (4.3) | 20 (8.2) | 12 (2.4) | 34 (14) | 17 (5.5) | 30 (11) |
| Orbital | 1.9 (0.9) | 8.7 (3.0) | 20 (4.4) | 32 (14) | 19 (4.3) | 34 (6.5) | 30 (10) | 25 (12) |
| Cingulate* | 26 (5) | 37 (9) | 45 (12) | 38 (4) | 35 (7) | 57a (4) | 47 (3) | 61a (4) |
| Piriform | 27 (8) | 31 (9) | 42 (5) | 36 (7) | 59 (12) | 71 (21) | 31 (18) | 50 (15) |
| Auditory* | 16 (7) | 162 (73) | 167 (63) | 365 (146) | 227 (39) | 477 (97) | 466 (195) | 494a (58) |
| Hypothalamus | ||||||||
| Dorsomedial nucleus | 0.5 (0.4) | 4.2 (1.1) | 7.6 (3.5) | 12 (4) | 12 (2) | 34 (9) | 24 (14) | 17 (9) |
| Lateral area* | 3.7 (1.2) | 12 (2) | 27a (6) | 13 (4) | 12 (5) | 21a (5) | 13 (2) | 29a (4) |
| Lateral preoptic area* | 1.4 (0.4) | 13a (2) | 18a (3) | 27b (5) | 81c (13) | 78c (15) | 89c (12) | 112c (17) |
| Medial preoptic area* | 2.5 (0.5) | 11a (3) | 27a (4) | 56b (8) | 48b (10) | 112c (37) | 164c (31) | 135c (29) |
| Medial preoptic nuc* | 2.6 (1.4) | 6.0 (1.2) | 12a (3) | 37b (11) | 26a (8) | 71b (18) | 95c (12) | 115c (19) |
| Paraventricular nuc* | 1.1 (0.5) | 15a (12) | 31a (10) | 56b (27) | 112b (41) | 125b (32) | 110b (23) | 161c (23) |
| Supramammillary nuc* | 0.7 (0.3) | 3.0a (1.4) | 21 (12) | 35b (17) | 27 (8) | 75c (26) | 98c (26) | 126c (34) |
| Ventromedial nucleus* | 0.3 (0.1) | 1.2a (0.5) | 1.5a (0.8) | 2.4a (0.9) | 10c (3) | 15c (3) | 12c (3) | 8.6c (3.0) |
| Thalamus | ||||||||
| Anterodorsal nucleus | 9.0 (4.4) | 12 (3) | 24 (14) | 30 (6) | 20 (6) | 32 (12) | 25 (7) | 29 (9) |
| Anteroventral nucleus | 2.4 (1.0) | 7.2 (2.7) | 21 (7) | 26 (5) | 19 (10) | 27 (8) | 25 (8) | 30 (8) |
| Central nuclei | 1.5 (0.4) | 10 (3) | 20 (7) | 13 (4) | 24 (12) | 31 (7) | 21 (6) | 35 (10) |
| Lateral genic. body | 43 (12) | 84 (26) | 79 (13) | 109 (24) | 136 (31) | 107 (37) | 78 (14) | 121 (38) |
| Medial genic. med* | 6.8 (2.6) | 14 (3) | 27 (7) | 21 (4) | 94c (12) | 74c (5) | 64b (9) | 93c (7) |
| Medial genic. ven/dor* | 35 (8) | 161a (34) | 246a (56) | 359b (111) | 412b (117) | 482b (59) | 476b (102) | 524b (97) |
| Ant. Paraventricular nuc* | 3.2 (1.3) | 12a (4) | 23a (9) | 16a (4) | 45c (17) | 61c (9) | 49c (13) | 62c (12) |
| Subparafasicular nuc* | 0.3 (0.1) | 22a (6) | 29a (7) | 20a (8) | 25a (9) | 55a (13) | 38a (69) | 33a (5) |
| Midbrain, pons, brainstem | ||||||||
| Cochlear nuclei* | 9 (4) | 142a (56) | 193a (26) | 298a (25) | 346b (17) | 376b (103) | 494b (140) | 491c (93) |
| Inferior coll. cen/dor* | 153 (17) | 413a (47) | 534a (68) | 649a (62) | 728b (42) | 768b (79) | 814b (94) | 886b (164) |
| Inferior coll. ext* | 54 (7) | 118a (27) | 109a (34) | 174a (53) | 176a (39) | 246b (34) | 299b (71) | 342c (53) |
| Sup. olivary complex* | 21 (4) | 146a (27) | 179a (13) | 191a (43) | 263a (34) | 323a (86) | 352a (43) | 403a (51) |
| Locus coeruleus* | 0.4 (0.1) | 1.1 (0.5) | 2.1 (0.3) | 1.2 (0.4) | 3.6a (0.5) | 2.8a (0.3) | 1.5 (0.2) | 2.6 (0.2) |
| Superior colliculus* | 13 (4) | 127a (32) | 58a (16) | 184a (36) | 259a (72) | 231a (58) | 296b (87) | 342b (46) |
| Cerebellum | ||||||||
| Flocculus/paraflocc.* | 84 (23) | 390a (106) | 461a (183) | 645a (106) | 817b (94) | 903b (148) | 916b (152) | 1104b (114) |
Notes to Table 1:
a
Significantly different from Control group (Tukey, P < 0.05).
b
Significantly different from Control and 80 dB groups (Tukey, P < 0.05).
c
Significantly different from Control, 80, 85 and 90 dB groups (Tukey, P < 0.05).
*
ANOVA results are significant (P < 0.05).