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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Exp Eye Res. 2008 Jan 17;86(4):653–660. doi: 10.1016/j.exer.2008.01.010

Figure 2. E2 regulates MMP-2 activity before and after oxidant injury in mouse RPE cell lines only in the presence of ERβ.

Figure 2

Representative zymograms of wildtype (A and B). ERKOα (D and E) and ERKOβ (H and I) RPE cell lines treated with 17β-estradiol (E2,1nM) for 24 hours before and after oxidant injury with MPO/H2O2 (I, injury). Supernatants were collected and electrophoresed on zymogram gels as described in methods. The upper zymograms (A, D and H) were loaded to visualize the pro-MMP-2 bands. The lower zymograms (B, E and I) were loaded to visualize the active MMP-2 bands. MMP-2 activity was measured by densitometry and % of vehicle control (v) determined. Data are graphed as mean ± SEM of the active band, + * p<0.05 compared to vehicle, # p<0.05 injury compared to E2 + injury, n=3. There was no protection against oxidant injury induced decreased MMP-2 activity in the absence of ERβ (ERKOβ cells). n=4. M=standard