ERKOα, ERKOβ and wildtype (data not shown) RPE cells were treated with E2 (1nM) for 24 hours before and after oxidant injury with MPO/H2O2 (I, injury) as described in methods. TIMP-2 activity (22kDa) was assessed on reverse zymography gels (inset), and densitometry performed. Shown is the data derived from ERKOα cell lines,**p<0.001 compared to vehicle (V), ##p<0.001 injury compared to injury +E2,