Fig. 7.

Unitary Cl⁻ turnover rate. (A) Traces from Cl⁻ electrode show the release of Cl⁻ into the liposome suspension. Arrows mark the addition of Vln/FCCP at the beginning of the experiment and 50 mM octylglucose at the end. Raw traces are shown (from the top to the bottom traces) for Cys-less, 433C, 230C/249C, 207C/207C, triple, and control with no protein reconstituted. All mutants were assayed after full cross-linking. (B) Turnover rates γ (filled bars) and f_o (open bars) for the indicated CLCs, each representing mean ± SE of five measurements.