Skip to main content
. 2007 Dec 17;104(52):20838–20843. doi: 10.1073/pnas.0706923105

Fig. 3.

Fig. 3.

Effect of gremlin on the activity and survival of AGM hematopoietic cells. (A–C) E11 AGMs cultured as explants in the presence or absence of gremlin (250 ng/ml) were analyzed after long term HSC repopulation assay (A), CFU-S11 assay (B), and CFU-C assay (C). For HSC repopulation, 0.3 AGM cell equivalents were injected per recipient. For each CFU-S11 condition, 14 mice were injected with 1 AGM equivalent (n = 3, *, P = 0.002). For CFU-C, n = 3. P < 0.01 for CFU-Mix. Data represent the mean ± SD. (D) FACS plots showing 7AAD and Annexin V staining of CD45+ cells from AGMs cultured in the absence or presence of gremlin (250 ng/ml). Percentages of cells shown in gated regions. (E) Graphs show absolute numbers of CD45+ cells and Annexin V+ 7AAD+CD45+ cells per AGM (n = 3). (F) Four-color staining dot plots showing percentages of viable cells from AGM explants cultured in the presence or absence of gremlin or Bmp4. (Upper) Cells gated within the CD45− population. (Lower) Cells gated within the total population. C, control; G, gremlin.