Table 1.
Effects of recombinant factors on AGM HSC repopulating activity
| Recombinant factor | Cells injected | Repopulated/transplanted (mice repopulated, %) | Donor chimerism range (mean, %) |
|---|---|---|---|
| None | 0.3 ee | 8/22 (36) | 27–100 (72) |
| β-NGF (25 ng/ml) | 0.3 ee | 3/8 (38) | 29–76 (58) |
| β-NGF (250 ng/ml) | 0.3 ee | 6/10 (60) | 25–100 (75) |
| MIP-1γ (20 ng/ml) | 0.3 ee | 8/13 (62) | 16–100 (75) |
| MIP-1γ (200 ng/ml) | 0.3 ee | 8/15 (53) | 22–100 (74) |
| None | 1 ee | 7/12 (58) | 37–77 (57) |
| Bmp4 (2 ng/ml) | 1 ee | 6/14 (43) | 23–72 (46) |
| Bmp4 (20 ng/ml) | 1 ee | 10/13 (77) | 21–69 (48) |
E11 AGM explants were cultured in the presence of recombinant factors. After 3 days of culture, AGM cells were collected, and 0.3 or 1 embryo equivalents (ee) of cells were injected into irradiated adult recipient mice. Four months after transplantation, mice were analyzed (>10% of donor chimerism is considered positive engraftment). n = 3 for MIP-1γ and Bmp4 and n = 1 for β-NGF.