Fig. 7.

Intra- and extracellular ROS of pH rescued rhd2-1 root hair. (A) Intracellular redox status of WT and rhd2-1 root hairs monitored with DCF. Higher DCF fluorescence intensity indicates higher cytoplasmic oxidative activity. Values are means ± SE of n = 16 (WT) and 23 (rhd2-1) root hairs. Means are significantly different (P < 0.001, Student t test). Fluorescence intensity was measured in a region of interest (ROI) in the apical cytoplasm (see Fig. 1). (B) ROS-dependent OxyBURST fluorescence along a pH-rescued growing rhd2-1 root hair. The image was acquired by using the same settings as for Fig. 3 A, B, and E. (Scale bar, 10 μm.) (C) Time course of extracellular ROS production of WT and rhd2-1 root hairs. OxyBURST fluorescence intensity (F.I.) was measured in an ROI behind the extreme apex. Data are representative of n = 14 experiments for WT and rhd2-1, respectively.