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. 2008 May 19;5:59. doi: 10.1186/1743-422X-5-59

Figure 3.

Figure 3

Western blotting analyses of the purified pregnancy plug mucins. Lane 1, MUC1 (positive control), lane 2, salivary MUC5B (negative control), lane 4, colonic mucus (positive control), lane 5, tracheal sputum (negative control), lane 7, pseudomyxoma peritonei (positive control), lane 8, salivary MUC7 (negative control), lane 10, pseudomyxoma peritonei (positive control), lane 11, gastric mucus (negative control) and lanes 3, 6, 9 and 12 purified pregnancy plug mucins were separated by a 1% agarose gel and transferred to nitrocellulose membrane. Following overnight blocking, the membranes were incubated for 2 h with mouse anti-MUC1 monoclonal (lanes 1, 2 and 3) and rabbit anti-MUC2 (lanes 4, 5 and 6), rabbit anti-MUC5AC (lanes 7, 8 and 9) and rabbit anti-MUC5B (lanes 10, 11 and 12) polyclonal antibodies. Membranes were then incubated for 1 h with HRPO linked goat anti-mouse and goat anti-rabbit secondary antibodies and bands that interacted with the antibodies were detected by ECL detection. NB the two bands of MUC2 (lane 6) are indicated by the arrows.