Figure 2.

Microarray analysis of LM15 binding to an array of plant cell wall polysaccharides and CDTA- and NaOH-solubilised isolates of Arabidopsis cell walls. Pectic and hemicellulosic polysaccharides from a range of species were microarrayed on nitrocellulose sheets and the relative binding of LM15 determined using an enzyme-linked secondary antibody detection system. Binding intensities were quantified and the horizontal scale indicates relative binding. The result shown is representative of at least three separate glycan profiling assays.