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RT-PCR demonstrates human 195-nucleotide (nt) IGFBP-3 products using cDNA transcribed from human colonic biopsy RNA. cDNA samples were prepared as described in Methods. GAPDH control (358 nt) is shown below for human samples. Controls at left are PCR products using IGFBP-3 standard (+ Control) and 'no reverse transcriptase (RT)' (-Control) cDNAs.
