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. 2008 Mar 12;28(11):2903–2911. doi: 10.1523/JNEUROSCI.0225-08.2008

Figure 3.

Figure 3.

CRH application reduces spine density in hippocampal organotypic slice cultures, whereas neurons grown in the presence of a CRH receptor antagonist have increased spine density. A, Apical dendrites of a CA3 pyramidal neuron in control condition. B, Treatment of hippocampal slices with CRH (100 nm; 13 d) decreased spine density (F(1,60) = 10.74; p = 0.001), an effect resulting primarily from lower spine density on the third- and fourth-order dendritic branches (*p < 0.05; n = 10 neurons per group; Bonferroni's post hoc test) (D). C, Growing hippocampal slices in the presence of the CRH receptor antagonist NBI 30775 (1 μm; 13 d) led to higher spine density (F(1,58) = 4.17; p = 0.046), also stemming from increased density of spines on the third- and fourth-order dendritic branches (*p < 0.05; n = 10 neurons per group; post hoc test) (D). The framed areas are magnified to show individual spines (arrowheads) and filopodia (C, arrow) on fourth-order branches. Hippocampi were cultured on P1 and grown for 14 d. Error bars indicate SEM. Scale bars: 55 μm (low magnification); 6 μm (high magnification).