Figure 6.
Spine disintegration induced by CRH requires activation of the receptor CRFR1. A, Application of the CRFR1 antagonist NBI 30775 (1 μm) for 30 min did not influence spine formation or elimination (F(1,36) = 0.1298; p = 0.7208, compared with untreated cultures). The red arrowheads denote newly formed spines, and the yellow ones show eliminated spines. B, Infusion of CRH (100 nm) accelerated spine disappearance (F(1,36) = 20.81; p < 0.0001). *p < 0.05, +p = 0.07, compared with formation at the same time point. C, This effect was blocked by infusion of the CRFR1 antagonist commencing 5–10 min before (and continuing during) CRH application (F(1,36) = 0.2529; p = 0.618, CRH+ antagonist compared with antagonist alone). Data are derived from five experiments, and 1367–2650 μm of dendrites were studied in each group. Organotypic cultures were initiated on P1 and grown for 5–6 d, followed by two-photon microscopy live imaging. Error bars indicate SEM. Scale bar, 11 μm.