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. 2008 May 28;105(22):7833–7838. doi: 10.1073/pnas.0802063105

Fig. 3.

Fig. 3.

FSP27 depletion has no effect on Cidea localization at the surface of lipid droplets in adipocytes. Immunofluorescence confocal microscopy displaying localization of endogenous Cidea and staining of lipid droplets with oil red in cultured brown adipocytes transfected with scrambled siRNA (a), transfected with FSP27 siRNA (b), and transfected with Cidea siRNA (c). In a and b the left three panels show a single optical plane of 4 μm each and the far right panels display the merged Z-sections. (Scale bar: 10 μm.) (d) Western blots showing the expression of FSP27 and Cidea in cultured brown adipocytes transfected with FSP27 or Cidea siRNA. (Left) Protein lysate from 3T3-L1 adipocytes was loaded as a positive control for FSP27. Actin was labeled as a loading control.