Skip to main content
. Author manuscript; available in PMC: 2008 Jun 4.
Published in final edited form as: Nature. 2007 Oct 28;450(7169):509–514. doi: 10.1038/nature06337

FIGURE 5. Function and evolutionary conservation of the CtIP C-terminus.

FIGURE 5

a. Alignment of CtIP and Sae2 arising from BLAST-searches with the CtIP C-terminus (790-897).

b-e, Deletion of the CtIP C-terminus impairs CtIP function. Three days after siRNA transfection, U2OS cells stably expressing GFP- tagged siRNA-resistant wild-type CtIP (WT) or a deletion mutant (1-789) were either treated with 1 μM CPT for 1 h and analyzed by immunoblotting or co-immunostaining, or were micro-irradiated and 30 min later co-immunostained as indicated. Asterisks in b and c: hyper-phosphorylated CtIP and RPA2.