Table 3. Identification of L-FABP.
MOWSE score | Number of matching peptides | Percent coverage | Protein MW (Da) | Protein pI | SwissProt Accession no. | Protein name |
---|---|---|---|---|---|---|
1045000 | 18 | 96 | 14 209 | 6.6 | P07148 | Liver fatty acid- binding protein |
462 | 4 | 32 | 18 013 | 7.7 | P05092 | Peptidyl-prolyl cis–trans isomerase A |
361 | 4 | 43 | 12 902 | 6.2 | P23763 | Vesicle-associated membrane protein 1 |
Example of an MS-Fit database search showing the identification of L-FAPB. The MOWSE score is much higher (225 times higher) for L-FABP compared with the next protein score. The ‘MOWSE score’ is a score based on how many peptides have been matched to the protein in the database and the accuracy of those matches, the higher the MOWSE score the better the hit; ‘no. of matching peptides’ is the number of experimentally obtained peptides that have been matched to theoretical peptides in the database; ‘Percent coverage’ is the percentage of the protein sequence covered by the matched peptides. ‘Protein MW and pI’ are the theoretical molecular weights and isoelectric points of the proteins; ‘Swissprot Accession no’ is an accession number in the SwissProt database that allows further information to be obtained about the identified protein; ‘protein name’ shows the name of the identified protein.