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. 2004 Apr 27;90(10):2032–2041. doi: 10.1038/sj.bjc.6601825

Figure 2.

Figure 2

Inhibition of purified soluble scFv 39, 40 and 69 binding on trastuzumab F(ab′)2 fragments by (A) HER-2/neu ECD-Fc fusion protein and (B) rhCEA, using a competitive ELISA. Increasing amounts of inhibitor were mixed with either anti-Id scFv 39, 40 or 69 used at a dilution giving an A490 ranging from 1 to 1.5 in ELISA (corresponding to a concentration of about 20 μg ml−1). The incubation on trastuzumab F(ab′)2 fragments for 1.5 h was followed by the detection of scFv binding with HRP-conjugated M2 anti-FLAG mAb (1 : 2000). The results obtained are expressed as percent inhibition at each concentration of inhibitor.