Figure 2.
Methylation-specific polymerase chain reaction of primary gastric cancers (T) and their corresponding non-neoplastic gastric mucosa (N). M, Chfr-methylated-sequence-specific PCR; U, Chf- unmethylated-sequence-specific PCR; P, positive control; DW, distilled water; and SM, size marker. Methylated Chfr is present in primary gastric cancers (M123, M137, M145, M157, M245), whereas non-neoplastic gastric mucosa samples do not exhibit methylated Chfr.