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. 2004 May 11;90(12):2370–2377. doi: 10.1038/sj.bjc.6601876

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Copyright © 2004 Cancer Research UK

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Cytochrome c release from mitochondria after treatment of NCI H460 cells with Mitoxantrone or cisplatin. Control transfected NCI H460 cells or NCI H460-Akt1 cells were grown in medium containing 0.5% serum overnight (-) and treated with 400 nM Mitoxantrone (Mito) or 20 μM cisplatin (CP) for 8 h, respectively. (A) Cell lysates were fractionally centrifugated and analysed with a cytochrome c ELISA as described in ‘Materials and methods’. Each value was determined as triplicate. (B) Aliquots of cell lysates were analysed by Western blotting with an antibody specific for caspase 8 as a control for cellular fractionation.