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| Fusion protein | CM79 bindinga (%) |
|---|---|
| MFECP (no tag) | 100 |
| MFEdmCP (no tag) | 0.8b |
| MFECPHis (His-tag) | 54b |
| MFEdmCPHis (His-tag) | 0.9b |
Measured by ELISA on CEA-coated plates. Binding of biotinylated CM79 was detected by Extravidin AP.
Binding of MFEdmCP, MFECPHis and MFEdmCPHis expressed as percentage of binding of MFECP.
