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. 2004 May 11;90(12):2364–2369. doi: 10.1038/sj.bjc.6601809

Table 1. Antibodies used for immunohistochemistry.

Protein detected Type of antibody source Antigen (amino acids) C- or N-terminus Immunohistochemistry: Frozen (F), paraffin (P)/dilution
NOS1 Monoclonal Synthetic human peptide (F)/1 : 100
  Transduction Lab C-terminus (1095–1289)  
       
NOS1 Polyclonal Rat recombinant protein (P)/1 : 200
  Zymed N-terminus (last 200 aa)  
       
NOS2 Polyclonal Synthetic human peptide (F)/1 : 500
  Santa Cruz C-terminus  
       
NOS3 Polyclonal Synthetic human peptide (F)/1 : 300
  Transduction Lab C-terminus  
       
sGC PolyclonalCayman Synthetic human peptide from α1 and β1 subunits (P)/1 : 300
       
3-NTY Polyclonal Nitrated KLH (P)/1 : 300
  Upstate Biotechnology