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. 2004 Jan 20;90(2):443–448. doi: 10.1038/sj.bjc.6601532

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Copyright © 2004 Cancer Research UK

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Expression of HER-2/neu RNA by real-time PCR (LightCycler). Amplification curve shows serial dilutions of an initial amount of SK-BR-3 RNA: 1 (3333 cells), 2 (333 cells), 3 (166 cells), 4 (33 cells), 5 (16 cells), 6 (1.6 cells) and 7 (0.6 cells). Inset, standard curve plot of the log of SK-BR-3 RNA cells numbers vs Cp. The standard curve shows seven orders of linear dynamic range (y=−3.67x+33.071; R²=0.9983).