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. 2004 Jan 20;90(2):535–541. doi: 10.1038/sj.bjc.6601510

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Copyright © 2004 Cancer Research UK

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Western blot analysis using specific antibodies for detection of p21, Rb and c-myc expression. The presented blots represent expression of the targets of sodium butyrate-treated PC3 human prostate cancer cells. Expression was evaluated at several time points after treatment (internal loading control not shown). P21 expression was induced by sodium butyrate with a maximum at 12 h post-treatment. Rb expression shifted to the hypophosphorylated form in a time-dependent pattern. Simultaneously, c-myc expression decreased. Molecular weights are given in kDa.