Table 2. Features of daughter ion spectra obtained by tandem mass spectrometry of molecular ions (M+H)+ of DMU 212 and four metabolites isolated from peaks in high-performance liquid chromatograms of extracts of livers of micethat had received DMU 212 (240 mg kg−1) and of peaks of authentic reference compounds.
| Authentic standard m/z | Liver extract m/z | |
|---|---|---|
| DMU 212 | 301 (M+H)+ (55) | 301 (M+H)+ (80) |
| 270 (100) | 270 (100) | |
| 286 (35) | 286 (50) | |
| 4,4′-Di-desmethyl-DMU 212 | 273 (M+H)+ (23) | 273 (M+H)+ (25) |
| 198 (100) | 198 (50) | |
| 181 (62) | 181 (81) | |
| 3-Desmethyl-DMU 212 | 287 (M+H)+ (10) | 287 (M+H)+ (75) |
| 227 (72) | 227 (20) | |
| 195 (70) | 195 (18) | |
| 4′-Desmethyl-DMU 212 | 287 (M+H)+ (10) | 287 (M+H)+ (60) |
| 256 (100) | 256 (100) | |
| 272 (80) | 272 (47) | |
| 3′-Hydroxy-DMU 212 | 317 (M+H)+ (31) | 317 (M+H)+ (21) |
| 225 (55) | 225 (100) | |
| 286 (22) | 286 (10) |
Only three prominent product ions are shown. Relative abundance in percentage is shown in parentheses.