Figure 4.

Effects of a protein phosphatase inhibitor, calyculin A, on GABA-induced whole-cell current responses in cerebellar Purkinje neurons. (A) Whole-cell membrane currents induced by bath-applied GABA (5 μM) before (left column) and after (right column) a 5-min perfusion of a saline containing calyculin A (1 μM) (Upper) or that containing vehicle alone (Lower). (B) Changes in peak amplitudes of GABA-induced currents of Purkinje neurons in calyculin A-treated (solid symbols, n = 8) and control (open symbols, n = 7) groups. Data points given in mean ± SEM. Current amplitudes were normalized with respect to the mean value recorded 8 min prior to bath application of the compounds (horizontal bar).