Figure 5.

Effects of intracellular injection of activated CaM-KII on GABA-induced whole-cell current responses in cerebellar Purkinje neurons. (A) Whole-cell membrane currents induced by bath-applied GABA (5 μM) 7 min (left column) and 17 min after (right column) initiation of whole cell recording in Purkinje neurons injected with activated (Upper) (200 nM) or heat-inactivated (Lower) (200 nM) CaM-KII. (B) Changes in average peak amplitudes of GABA-induced currents of Purkinje neurons injected with activated CaM-KII (solid symbols, n = 6) and those with heat-inactivated enzyme (open symbols, n = 5). Data points given in mean ± SEM. The ratio of the peak GABA-induced current at indicated time (It) was divided by the peak GABA-induced current attained 5 min after patch formation (I5).