Figure 1.

Northern blot of total cellular RNA (20 μg) separated in a 1% agarose/formaldehyde gel from the normal human pancreas tissues, CD18/HPAF cultured cells, and the CD18/HPAF cells grown as SC and OT tumours. (A) Blot was probed with a ³²P-labelled MUC4 tandem repeat cDNA probe, and the same membrane was stripped and hybridised with a GAPDH cDNA probe. (B) Densitometric values (±s.e.) for the bands above in three different experiments were determined by using Molecular Dynamics ImageQuant software program. Values obtained for the MUC4 smear were divided by the densitometic values for the GAPDH band.