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. 2004 Feb 3;90(3):657–664. doi: 10.1038/sj.bjc.6601604

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Copyright © 2004 Cancer Research UK

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(A) Northern blot of total cellular RNA (20 μg) extracted from SC tumour cells, OT tumour cells at different passages and CD18/HPAF cell line, separated in a 1% agarose/formaldehyde gel. (a) Probed with ³²P-labelled MUC4 tandem repeat cDNA probe. (b) The same membrane as shown in (a), probed with a GAPDH cDNA probe. (B) Densitometric values (±s.e.) for the bands in three different experiments were determined by using Molecular Dynamics ImageQuant software program. Values obtained for the MUC4 smear were divided by the densitometric values for the GAPDH band.