Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Mar 2;90(5):1100–1107. doi: 10.1038/sj.bjc.6601640

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of yessotoxin treatment of MCF-7 cells on the levels of β- and γ-catenins associated with E-cadherin. MCF-7 cells were treated with (+) or without (−) 1 nM YTX for 5 days at 37°C. At the end of the incubation, cells were processed to prepare cytosoluble extracts, which were subjected to immunoprecipitation using the HECD-1 anti-E-cadherin antibody, as described under Materials and Methods. Identical aliquots of cytosoluble extracts (CYT) and of immunoprecipitated material (IPPT) were subjected to SDS–PAGE and immunoblotting, using antibodies recognising the indicated proteins. The electrophoretic mobilities of β-galactosidase (116 kDa) and fructose-6-phosphate kinase (90 kDa) subunits, used as marker proteins running in a parallel lane, are indicated on the left.